biohybrid
 
 
 
 
 
 
 
 
 
 
 

Publications + Presentations


Papers

 

Allodi, I., Udina, E., Navarro, X.(2012): Specificity of peripheral nerve regeneration: Interactions at the axon level; Progress in Neurobiology 98 (2012) 16-37

 

Allodi I., Casals-Díaz L., Santos-Nogueira E., Gonzalez-Perez F., Navarro X., Udina E. FGF-2 low molecular weight selectively promotes neuritogenesis of motor neurons. Mol Neurobiol 2013, 47:770-781.

 

Allodi I., Mecollari V., González-Pérez F., Eggers R., Hoyng S., Verhaagen J., Navarro X., Udina E. Schwann cells transduced with a lentiviral vector encoding Fgf-2 promote motor neuron regeneration following sciatic nerve injury. Glia 2014, 62:1736-46.

 

Buskbjerg Jager S, Ronchi G., Bjerggaard Vaegter C, Geuna S. The mouse median nerve experimental model in regenerative research. Biomed Res Int. 2014;2014:701682.

 

Chemnitz A., Dahlin LB., Carlsson IK. Consequences and adaptation in daily life - patients' experiences three decades after a nerve injury sustained in adolescence. BMC Muscoloskel Dis 2013, 22(14):252.

 

Cobianchi S., de Cruz J., Navarro X. Assessment of sensory thresholds and nociceptive fiber growth after sciatic nerve injury reveal the differential contribution of collateral reinnervation and nerve regeneration to neuropathic pain. Exp Neurol 2014, 255:1-11.

 

Gambarotta G., Ronchi G., Geuna S., Perroteau I. Neuregulin 1 isoforms could be an effective therapeutic candidate to promote peripheral nerve regeneration. Neural Regen Res. 2014 15;9(12):1183-5.

 

Gambarotta G., Ronchi G., Friard O, Galletta P, Perroteau I., Geuna S. Identification and validation of suitable housekeeping genes for normalizing quantitative real-time PCR assays in injured peripheral nerves. PLoS One. 2014 Aug 21;9(8):e105601.

 

Gonzalez-Perez F., Cobianchi S., Geuna S., Barwig C., Freier T., Udina E., Navarro X. Tubulization with chitosan guides for the repair of long gap peripheral nerve injury in the rat. Microsurg in press.

 

Haastert-Talini, K., Geuna, S., Dahlin, L. B., Meyer, C., StenbergL., Freier, T, Heimann, C., Barwig, C.,  Pinto, L. F.V., Raimondo, S., Gambarotta, G., Samy, S. R., Sousa, N., Salgado, A. J., Ratzka, A., Wrobel, S., Grothe, C. (2013): Chitosan tubes of varying degrees of acetylation for bridging peripheral nerve defects. Biomaterials 34 (2013), pp. 9886-9904

 

Hallgren A., Björkman A., Chemnitz A., Dahlin L.B.. Subjective outcome related to donor site morbidity after sural nerve graft harvesting: a survey in 41 patients. BMC Surg. 2013, 13:39.


Morano M.*, Wrobel S.*, Fregnan F., Ziv-Polat O., Shahar A., Geuna S., Grothe C., Haastert-Talini K.
Nanotechnology versus stem cell engineering - In vitro-comparison of neurite inductive potentials. Int J Nanomedicine 9(1):5289-5306


Oliveira N.G., Sirgado T., Reis L., Pinto L.F.V., da Silva C.L., Ferreira F.C., Rodrigues A. In vitro assessment of three dimensional dense chitosan-based structures to be used as bioabsorbable implants, Journal of the Mechanical Behavior of Biomedical Materials 40 2014, 413–425.


Oliveira N.G., Rodrigues A., Reis L., Pinto L.F.V. Trends in Bioabsorbable Osteosynthesis Devices: Introduction to a Novel Production Process of Chitosan-Based Implants, J. Chitin Chitosan Sci. 2013, Vol. 1, No. 3, 1-11.

 

Stenberg, L., Kanje, M., Dolezal, K., Dahlin, L.B.(2012): Expression of activationg transcription Factor 3 (ATF 3) and caspase Schwann cells and axonal outgrowth after sciatic nerve repair in diabetic BB rats; Neuroscience letters 515 (2012) 34-38

 

Stenberg L., Dahlin L.B.. Gender differences in nerve regeneration after sciatic nerve injury and repair in healthy and in type 2 diabetic Goto-Kakizaki rats. BMC Neurosci. 2014, 15:107.

 

Wrobel S., Serra S.C., Ribeiro-Samy S., Sousa N., Heimann C., Barwig C., Grothe C., Salgado A.J., Haastert-Talini K. (2014): In Vitro Evaluation of Cell-Seeded Chitosan Films for Peripheral Nerve Tissue Engineering. Tissue Engineering Part A

 

Ziv-Polat O, Shahar A, Levy I, Skaat H, Neuman S, Fregnan F, Geuna S, Grothe C, Haastert-Talini K, Margel S. (2014):  The role of neurotrophic factors conjugated to iron oxide nanoparticles in peripheral nerve regeneration - in vitro studies. BioMed Research International, Vol 2014 (2014)

 

Letter to the Editor: "BIOHYBRID-Biohybrid templates for peripheral nerve regeneration", Grothe et al. , Journal of the Peripheral Nervous System 17:220–222 (2012)



Book Chapters

 

 

Geuna S, Haastert-Talini K, Navarro X. (2013): The nerve trunk in injury and during regeneration. In Dahlin L, Ada S, Leblebicioğlu G (eds): Current treatment of nerve injuries and disorders. Federation of European Societies for the Surgery of the Hand. Palme Publications

 

Gnavi S, Barwig C, Freier T, Haastert-Talini K, Grothe C, Geuna S              
Chapter 1: The Use of Chitosan-Based Scaffolds to Enhance Regeneration in the Nervous System
In: Int Rev Neurobiol. 2013;109C:1-62.

 

Shahar A., Ziv-Polat O., Margel S., and Freier T. Developing a Customized Tissue-Engineered Implant for Peripheral Nerve Reconstruction: Role of Nanoparticles. In: Bhoop BS, ed. Volume 5: Medical Nanobiotechnology of the Series "Nanobiomedicine". USA: Studium Press LLC 2014.

 

 

 



Published abstracts

 

Haastert-Talini, K., Geuna, S., Dahlin, L.B., Salgado, A.S., Shahar, A.,Freier, T., Grothe, C., and the BIOHYBRID consortium. New chitosan-based scaffolds for peripheral nerve regeneration – pre clinical studies. 3rd International
Conference on Tissue Science & Regenerative Medicine, September 24th-26th, Valencia, Spain, J Tissue Sci Eng 2014; 5(3):35


Wrobel, S., Morano, M., Meyer, C., Shahar, A., Ziv, O., Haastert-Talini, K., Geuna, S., Grothe, C. Development of cell-enhanced chitosan scaffolds to overcome long gaps after peripheral nerve injury. Cytotherapy 2014, Vol. 16, Issue 4, Supplement, Page S102

 

 



Conference contributions

 

Carvalho C.R., Goncalves J.P., Reis R.L., Oliveira J.M., TermStem 2014, 23-24 October 2014, Porto, Portugal. Application of keratin/chitosan conduits for peripheral nerve regeneration.

 

Freier, T., 18th DGH Fortbildungsseminar, 05. – 06. September 2014 Berlin, Germany. Presentation of Reaxon® Nerve Guide (Congress booth)

 

Arbat-Plana A., Macias-Gómez A., Navarro X., Udina E., 9th FENS Forum of Neuroscience, July 2014, Milan, Italy. Time course characterization and effects of NT3/BDNF application on the synaptic stripping suffered by rat motoneurons after sciatic nerve injury. 

 

Gonzalez-Perez F., Alé A., Navarro X., Udina E., 9th FENS Forum of Neuroscience, July 2014, Milan, Italy. In vitro comparison of motor and sensory neuron outgrowth in different extracellular matrix (ECM) enriched matrices.

 

Morano, M., Fregnan, F., Wesemann, M., Ziv-Polat, O., Shahar, A., Grothe, C., Haastert-Talini, K., Geuna S., 9th FENS Forum of Neuroscience, July 2014, Milan, Italy. In vitro analysis on NVR-gel combined with iron oxide-conjugated factors for peripheral nerve engineering.

 

Dahlin L.B., 5th Regenerative Medicine Symposium “Regeneration of Nerves and Epithelia: Current Progress & Future Therapies”June 26th-27th 2014, Lübeck, Germany. Clinical challenges in nerve reconstruction.

 

Grothe, C., 5th Regenerative Medicine Symposium “Regeneration of Nerves and Epithelia:  Current Progress & Future Therapies”, June 26th-27th 2014, Lübeck, Germany. Biohybrid nerve devices

 

Meyer C., Haastert-Talini K., Wrobel S., Grothe C., 5th Regenerative Medicine Symposium “Regeneration of Nerves and Epithelia: Current Progress & Future Therapies”, June 26th-27th 2014, Lübeck, Germany. Addressing the long gap: Reaxon nerve guides supplemented with genetically modified Schwann cells over- expressing GDNF or FGF-218kDa in NVR-Gel.

 

Stenberg L., Geuna S., Grothe C., Freier T., Dahlin L.B., 5th Regenerative Medicine Symposium “Regeneration of Nerves and Epithelia: Current Progress & Future Therapies”, June 26th-27th 2014, Lübeck, Germany, Reconstruction of sciatic nerve defect in healthy and diabetic GK rats with hollow chitosan tubes (BIOHYBRID) and autologous nerve grafts – short time perspectives.

 

Carvalho C.R., Pedro J., Ng K.W., Neves N., Reis R.L., Oliveira J.M., TERMIS-EU 2014 Chapter Meeting, 10-13 June 2014, Genova, Italy. Keratin/chitosan as novel grafts for peripheral nerve regeneration.

 

Geuna, S., Haastert-Talini, K., Freier, T., Grothe, C., and the BIOHYBRID Consortium TERMIS-EU 2014 Chapter Meeting, 10-13 June 2014, Genova, Italy. Different acetylation of chitosan conduits for the peripheral nerve repair.

 

Wrobel S., Morano M., Meyer C., Shahar A., Ziv-Polat O., Haastert-Talini K., Geuna S., Grothe C., 20th ISCT Annual Meeting, April 23rd-26th 2014, Paris, France; Cytotherapy, 16 (4), Supplement, April 2014 Development of cell-enhanced chitosan scaffolds to overcome long gaps after peripheral nerve injury.

 

K. Haastert-Talini and BIOHYBRID consortium, 5th Vienna Symposium on Surgery of Peripheral Nerves, March 21.-23., 2014, Vienna, Austria. BIOHYBRID - development of new chitosan based nerve graft - first pre-clinical results.”

 

Stenberg L., Kodama A., Freier T., Geuna S., Dahlin L.B., 5th Vienna Symposium on Surgery of Peripheral Nerves, March 21.-23., 2014, Vienna, Austria. Nerve regeneration after nerve repair or after nerve reconstruction with hollow chitosan conduits (BIOHYBRID) or with autologous nerve grafts bridging a sciatic nerve defect in healthy and diabetic Goto-Kakizaki (GK) rats.

 

The BIOHYBRID Consortium presented extensive insights into the ongoing research at the 2nd international Symposium on Peripheral Nerve Regeneration (ISPNR), Jan. 23-25th, 2014 in Turin, Italy.

 

Carvalho, C.R., Silva-Correia, J., Martins, A.,  Neves, N.M., Oliveira, J.M., Reis, R.L.,  Chitosan nanofibers for guided peripheral nerve regeneration

 

Cobianchi S., Navarro X. Advantages and pitfalls of algesimetry tests for functional evaluation after nerve injuries.

 

Gonzalez-Perez, F., Cobianchi. S., Barwig, C., Freier, T., Udina E., Navarr, X.,  Tubulization with chitosan guides for the repair of long gap peripheral nerve injury in the rat

 

Haastert-Talini, K., Grothe C., Geuna, S., Dahlin, L.B., Salgado, A., Freier, T. The BIOHYBRID project:  pre-clinical evaluation of chitosan-based artificial nerve grafts.

 

Iannello A., Ronchi G. , Pascal D., Crosio A., Friard O., Geuna S., Gambarotta G. Expression analysis of Neuregulin 1 isoforms and ErbB receptors in two experimental models : end-to-end repair and crush injury.

 

Meyer, C., Haastert-Talini, K., Wrobel, S., Ratzka, A., Grothe, C., Inflamatory and physiological tissue responses following nerve reconstruction with chitosan tubes of different degrees of acetylation  (Poster)

 

Morano, M., Fregnan, F., Wesemann, M., Ziv-Polat, O., Shahar, A., Grothe, C., Haastert-Talini, K., Geuna, S.,  In vitro evaluation of NVR-gel enriched with neurotrophic factors conjugated to iron oxide nanoparticles for peripheral nerve repair (Poster)

 

Navarro X. Electrophysiological and functional tests: what is meaningful for functional recovery.

 

Shapira, Y., Tolmasov, M., Shchetinkov, L., Nissan, M., Reider, E., Koren, A., Biron, T., Bitan, Y., Ronchi, G., Geuna, S.,  Rochkind, S., Peripheral nerve reconstruction of sciatic nerve in rats using chitosan hollow tubes versus standard care with nerve graft

 

Silva N.A., Gomes E.D., Silva R.C., Serra, S.C., Wrobel, S., Grothe, C., Haastert-Talini, K., Sousa, N., Salgado, A.J., Peptide grafted gellan gum based hydrogels and mesenchymal stem cells in peripheral nerve regeneration

 

Stenberg, L., Kodam, A., Freier, T., Geuna, S., Dahlin L.B. Nerve regeneration in hollow chitosan tubes (BIOHBYBRID) and in autologous nerve grafts bridging a sciatic nerve defect in healthy and diabetic GK rats.

 

Wrobel, S., Wesemann, M., Grothe, C., Heimann, C., Shahar, A., Ziv-Polat, O., Salgado, A., Haastert-Talini, K., In vitro evaluation of cell-seeded chitosan tubes for peripheral nerve tissue engineering (Poster)

 

Ziv-Polat, O., Geuna, S., Grothe, C., Margel, S., Shahar, A., Role of nerve growth factors conjugated to iron oxide nanaoparticles in the reconstruction of severed perheral nerve- in vitro studies

 

Freier, T.  Presentation of Reaxon® Nerve Guide. (Congress booth)

 

Raimondo S., Geuna S., Haastert-Talini K., Freier T., Grothe C., and the BIOHYBRID Consortium, 15th International Symposium on Neural Regeneration, December 2013, Pacific Grove, California, USA. A new chitosan scaffold for peripheral nerve repair.

 


Stenberg L., Geuna S., Grothe C., Freier T., Dahlin L.B. Neuroscience, November 2013, San Diego USA Nerve regeneration in hollow chitosan tube (BIOHYBRID) and in autologous nerve grafts bridging a sciatic nerve defect in healthy and diabetic rats.

 

Geuna, S., Haastert-Talini, K., Freier, T., Grothe, C., and the BIOHYBRID Consortium 2013 annual meeting of the Asia Pacific Chapter of the Tissue Engineering and Regenerative Medicine International Society (TERMIS), October 23rd – 26th 2013, Shanghai, China. Pre-clinical assessment of chitosan scaffolds of varying degrees of acetylation for repairing peripheral nerve lesions with substance loss. 

 

Carvalho CR, Silva-Correia J, Martins A, Oliveira JM, Reis RL. TermStem 2013, 10-12 October 2013, Porto, Portugal. Chitosan nanofibers as scaffolds for peripheral nerve regeneration.  

 

Reider E., Rochkind S., 15th World Congress of Neurosurgery (WFNS), 8-13 September, 2013, Seoul, Korea. Monitoring of rat sciatic nerve and chitosan tube following complete nerve injury.

 

González-Pérez F., Allodi I., Navarro X., Udina E.  15 Congreso Nacional de la  Sociedad Española de Neurociencia, 25-27 September 2013, Oviedo, Spain. Schwann cells transfected with a lentiviral vector overexpressing  FGF-2: in vitro and in vivo effects on to preferentially promote motorneuron regeneration.


Stenberg L., Dahlin L.B. FENS, Prague, 11-14 September, 2013. Nerve regeneration is different in male and female in healthy and diabetic rats after nerve repair.

 

Pinto, L.V.F, Prata S., Espiga A., Oliveira, N.G., Pires E. EUCHIS 2013 – 11th International Conference of the European Chitin Society, 5th-8th May, Porto, Portugal, 2013. From medical grade chitosan production to medical devices manufacture and commercialization.

 

Stenberg L., Kanje M., Dahlin L.B. FESSH, May 29 – June 1, 2013, Antalya, Turkey. Gender differences in nerve regeneration after sciatic nerve injury and repair in healthy and diabetic rats.

 

K. Haastert-Talini and BIOHYBRID consortium German Society for Neurosurgery (DGNC) section "peripheral nerves”, Feb 11th 2012, Hannover, Germany,EU-FP7-HEALTH-2011-278612 Biohybrid templates for peripheral nerve regeneration”

 

S. Geuna and BIOHYBRID consortium 11th Congress of the European Federation of Societies of Microsurgery (E.F.S.M), April 5th – 7th 2012, in Paris, France.Biohybrid templates for peripheral nerve regeneration” 

 

S. Geuna, Lars B. Dahlin11th Congress of the European Federation of Societies of Microsurgery (E.F.S.M), April 5th – 7th 2012, in Paris, France. “Current concepts in Experimental Microsurgery” –  Advances in peripheral nerve tissue engineering”.

 

Haastert-Talini K., Grothe C., BIOHYBRID Consortium (2012) 8th FENS Forum of Neuroscience, Jul 14th – 18th 2012, Barcelona, Spain. FENS Abstract, Volume 6, p012.35, 2012 Biohybrid Templates To Increase Peripheral Nerve Regeneration. 

 

K. Haastert-Talini, Collaborators and BIOHYBRID consortium 3rd TERMIS World Congress 2012, Sept 5th – 8th 2012, Vienna, Austria. “Keynote: New molecules and biohybrid templates to increase peripheral nerve regeneration”. (J Tissue Eng Reg Med 2012; 6 (Suppl.1): 83)

 

K. Haastert-Talini, C. Grothe 46th DGBMT Annual Meeting, BMT 2012, Sept 16th – 19th 2012, Jena, Germany. “Best Practice: Coordination of EU-Project: Biohybrid Templates for Peripheral Nerve Regeneration”. 

 

Cobianchi S, Casals-Diaz L, Udina E, Jaramillo J, Navarro X 8th FENS Forum of Neuroscience, Jul 14th – 18th 2012, Barcelona, Spain. “Effects of early treadmill exercise, electrical stimulation and their combination on neuropathic pain and neurotrophins expression after peripheral nerve injury”

 

Allodi I, Mecollari V, González-Pérez F, Eggers R, Hoyng S, Casas C, Navarro X, Verhaagen J, Udina E 8th FENS Forum of Neuroscience, Jul 14th – 18th 2012, Barcelona, Spain. “In vitro and in vivo characterization of lentiviral vector overexpressing FGF-2 – a promising tool to promote peripheral nerve regeneration”.

 

Talk by UNITO: S. Geuna and the BIOHYBRID Consortium CEITEC International Conference – Cell Interaction with Surfaces, October 22-23, 2012, Brno, Czech Republic. "Biohybrid templates for enhancing peripheral nerve repair and regeneration",

 

Udina E, Allodi I, Casals L, Hernández J, Roet KCD, Navarro X. Society for Neuroscience 2011, Nov 12th-16th 2011, Washington DC, USA. “Role of cell-axon interaction in specific motor and sensory regeneration”. 

 

Allodi I, Casals-Díaz L, Mason M, Van Kesteren R, Verhaagen J, Navarro X, Udina E Society for Neuroscience 2011, Nov 12th-16th 2011, Washington DC, USA. “FGF-2 (low molecular weight - 18 kDa) specifically promotes neuritogenesis in motor neurons in vitro”.